Induction of Resistance by Listeria monocytogenes Cell Wall Fraction
نویسندگان
چکیده
منابع مشابه
Induction of Resistance by Listeria monocytogenes Cell Wall Fraction.
A crude cell wall fraction of Listeria monocytogenes was prepared by sonic disruption and differential centrifugation of viable, washed cultures. When injected into mice, this sterile, crude cell wall fraction protected mice against an intraperitoneal challenge with 18 to 85 50% mean lethal dose of L. monocytogenes. Resistance was greatly enhanced when bacterial endotoxin (lipopolysaccharide) w...
متن کاملRemodeling of the Listeria monocytogenes cell wall inside eukaryotic cells
Listeria monocytogenes is an intracellular Gram-positive bacterial pathogen that produces many types of surface proteins. To get insights into its intracellular lifestyle, we used high-resolution mass spectrometry to characterize the cell wall proteome of bacteria proliferating within the eukaryotic cell. The relative amount of a few surface proteins was found notoriously different in intracell...
متن کاملIntracellular induction of Listeria monocytogenes actA expression.
Following entry into the host cytosol, the bacterial pathogen Listeria monocytogenes dramatically increases the expression of several key virulence factors. The expression of actA, whose protein product is required for L. monocytogenes actin-based intracellular motility, is increased by more than 200-fold in cytosolic bacteria in comparison to broth-grown cultures. Two distinct promoter element...
متن کاملAssociation of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes.
Listeria monocytogenes is a Gram-positive intracellular bacterial pathogen that colonizes the cytosol of eukaryotic cells. Recent transcriptomic studies have revealed that intracellular L. monocytogenes alter expression of genes encoding envelope components. However, no comparative global analysis of this cell wall remodeling process is yet known at the protein level. Here, we used high resolut...
متن کاملListeria monocytogenes cell walls induce decreased resistance to infection.
A significant decrease in murine resistance to Listeria monocytogenes was induced by using crude Listeria cell wall fraction (LCWF) and purified Listeria cell walls (PF). When equal amounts of these materials were injected, PF was more effective than LCWF in decreasing resistance. The PF effect was dose dependent when measured either as a decrease in 50% lethal dose of the Listeria challenge or...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1974
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.10.5.1163-1169.1974